Fig. 3.

Rab5 is required for TUFT1-dependent Rac1 activation in TNBC cells. a TUFT1 expression was downregulated by TUFT1-shRNA and upregulated by PLNCX2-TUFT1 in MDA-MB-231 and HCC1937 cells. Rac1-GTP expression was examined by western blotting (n = 3). b Rab5-shRNA or scr-shRNA were transfected into TUFT1-overexpressing MDA-MB-231 and HCC1937 cells. Rac1-GTP expression was examined by western blotting (n = 3). c Endogenous interaction between TUFT1 and p85α in MDA-MB-231 cells by co-immunoprecipitation. d, e PLNCX2-p85α or PLNCX2-Vector were transfected into TUFT1-overexpressing MDA-MB-231 cells. d Rab5-GTP expression was examined by western blotting (n = 3). e Cell migration was monitored by transwell assay (n = 3). Results are presented as mean ± SD. The statistical significance was assessed by student’s t-test; **p < 0.01