Fig. 1.

Quantitative analysis of kirenol and darutoside in Sigesbeckia glabrescens Makino (SG). HPLC chromatograms of a SG extract (1 mg/mL) and b mixed standards (7.5 μg/mL of kirenol and darutoside). 1: kirenol; 2: darutoside. c Chemical structure of kirenol. d Chemical structure of darutoside. RAW 264.7 cells were pretreated with e kirenol (0, 25, 50, and 100 μg/mL) or f darutoside (0, 25, 50, and 100 μg/mL) for 4 h followed by the addition of lipopolysaccharides (LPS, 1 μg/mL) for 12 h. Nitric oxide (NO) release was detected using the Griess reagent