Skip to main content
. 2019 Sep 12;10:1025. doi: 10.3389/fphar.2019.01025

Figure 5.

Figure 5

TSN pretreatment decreased synthesis of influenza virus A/PR/8/34 mRNA but did not affect the antiviral host response. (A) MDCK cells were pretreated with TSN (2 µM) as described then infected with 10 MOI A/PR/8/34. Expression levels of HA, NP, PA, and M2 viral mRNAs were assessed by qRT-PCR. (B) Host expression levels of IFN-β and Mx1 were assessed at 3 and 18 hpi by qRT-PCR. Expression levels were normalized to β-actin mRNA. Data are presented as mean ± SD of three replicates and are representative of three independent experiments (n.d., not detected). V, control virus-infected MDCK cells. Statistical significance was analyzed by Student’s t-test. **P < 0.01; ***P < 0.001.