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TSN pretreatment inhibited the phosphorylation of AKT induced by influenza A virus infection. MDCK cells were pretreated with TSN at 2 µM for 6 h prior to infection by 10 MOI A/PR/8/34. Cells were stained with anti-phospho-AKT antibody (left column, green) and counterstained with DAPI (middle column, blue) at 18 hpi. The phospho-AKT protein and DAPI image are merged (right column). Data are representative of three independent experiments. Scale bars, 100 µm at ×20 magnification.
