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. 2002 Apr 15;22(8):3090–3099. doi: 10.1523/JNEUROSCI.22-08-03090.2002

Fig. 3.

Fig. 3.

Expression of α-synuclein and TH in parental and in stably transfected MN9D clonal cell lines. a, Parental MN9D (lane 1) and GFP-transfected MN9D cells (lane 2) express low endogenous α-synuclein levels compared with clonal cells overexpressing wild-type α-synuclein (WT Syn, lanes 3,4), or A53T mutant α-synuclein (lanes 5, 6) as determined by immunoblot of 20 μg protein per lane reacted with the Syn-1 anti-α-synuclein antibody. b, TH levels are equivalent in all MN9D cell lines whether expressing endogenous α-synuclein (parentalMN9D, lane 1, or GFP-MN9D,lane 2) or overexpressing α-synuclein [wild-type (WT), lanes 3 and4, or A53T, lanes 5 and6] as determined by immunoblot of 20 μg protein per lane reacted with the MAB318 anti-TH antibody.