Fig. 2.

All recorded cells had the expected physiological properties of precursor cells. A, Typical recordings of current responses to a series of voltage steps in voltage-clamp mode demonstrate low input resistance. B, Current–voltage is linear between −90 mV and +10 mV in the same cell as A.C, Current responses to a series of voltage steps recorded in current-clamp mode. D, Leak-subtracted voltage-clamp recordings confirm the absence of voltage-gated sodium conductances. E, Representative cells recorded and filled at E12, E15, E16, and E18. Five representative recorded cells shown atE18 exhibit radial glial morphology, with an endfoot on the ventricle, a cell body within the VZ, and a radial process extending to the pia (P). Scale bar, 10 μm.