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. 2002 Apr 15;22(8):3070–3080. doi: 10.1523/JNEUROSCI.22-08-03070.2002

Fig. 2.

Fig. 2.

GABAA receptor-mediated IPSCs recorded in PCC/RSC pyramidal cells. Whole-cell recordings were performed using a CsCl-based internal solution at holding potentials of −70 mV and +30 mV. When the holding potential was −70 mV (A), inward currents were recorded. When the cell was held at +30 mV, the currents became outward (B). The GABAA receptor antagonist BMI (20 μm) abolishes the currents (C). In the presence of BMI (20 μm) and at the holding potential of −70 mV, fast inward currents are recorded from another pyramidal cell and can be blocked by DNQX (20 μm) (D). In the presence of d-AP5 (50 μm) and DNQX (20 μm), amplitudes of evoked IPSCs recorded from another pyramidal cell held at potentials ranged from −70 to +20 mV (E). An I–V curve was constructed (F) based on the evoked synaptic responses shown in E. The apparent reversal potential was approximately −39 mV. These findings indicate that the recorded IPSCs were mediated by GABAA receptors. Calibration:A–C, 500 msec, 0.1 nA; D, 100 msec, 50 pA.