Fig. 8.

RT-PCR analyses from RNA preparations taken from untreated slices as well as from slices treated with antisense (as), mixed base ODNs (mb), DNA enzyme (DNA enz), and mixed base DNA enzyme (DNA mb). The slices (treated and untreated) were maintained in culture for 7 d for antisense and mixed base ODNs and for 4 d for DNA enzyme and control DNA enzyme. A, RT-PCR Southern blot for the γ1 chain of laminin after antisense and mixed base ODN treatment (expected RT-PCR product size, 450 bp).B, Agarose gel of an RT-PCR for β-actin mRNA from the same preparations shown in A (expected RT-PCR product size, 260 bp). Note that the laminin γ1 chain mRNA shows only minimal changes, which are repeated in the size of the actin bands.C, RT-PCR Southern blot for the γ1 chain of laminin from slice cultures treated with DNA enzyme showing a reduction of γ1 chain mRNA compared with slices treated with control DNA enzyme (expected RT-PCR product size, 450 bp). D, Agarose gel of an RT-PCR for β-actin mRNA from the same preparation as shown inC (expected RT-PCR product size, 260 bp).E, The use of the same preparation as inC for an RT-PCR of the laminin β2 chain (expected RT-PCR product size, 349 bp). This related chain to the γ1 chain of laminin is not affected by the DNA enzyme treatment. The PCR control is an RT-PCR without RNA but with all buffers to check for contamination.