Table 2.
In situ hybridization histochemistry for UCH-L1 mRNA expression after CCI
| Laminas | Mediolateral | Lateral | ||||
|---|---|---|---|---|---|---|
| CCI/ipsilateral | CCI/contralateral | Naive | CCI/ipsilateral | CCI/contralateral | Naive | |
| Mean number of hybridizing cells per section in spinal dorsal horn | ||||||
| LI | 35.5 ± 1.2* | 26.6 ± 0.5 | 25.9 ± 1.1 | 32.2 ± 1.4* | 24.4 ± 1.1 | 22.5 ± 2.5 |
| LII | 35.2 ± 1.8* | 25.5 ± 1.2 | 25.5 ± 1.4 | 34.2 ± 1.8* | 28.2 ± 2.1 | 30.1 ± 1.3 |
| LIII | 37.1 ± 1.9* | 30.5 ± 1.2 | 30.5 ± 1.5 | 36.5 ± 3.1 | 32.5 ± 2.5 | 35.6 ± 2.2 |
| LIV | 42.2 ± 2.2 | 38.2 ± 3.2 | 38.8 ± 3.4 | 41.1 ± 2.1 | 40.5 ± 1.4 | 40.1 ± 1.3 |
| LV | 38.9 ± 1.4 | 36.5 ± 1.8 | 37.5 ± 2.2 | 32.5 ± 1.2 | 32.1 ± 0.6 | 32.4 ± 1.0 |
| Mean number of silver grains per cell | ||||||
| LI | 14.5 ± 1.3* | 10.3 ± 0.8 | 8.8 ± 1.3 | 12.4 ± 1.3* | 9.2 ± 1.2 | 8.8 ± 0.4 |
| LII | 14.5 ± 1.2* | 11.6 ± 0.8 | 11.1 ± 1.2 | 13.6 ± 1.2* | 10.3 ± 0.9 | 10.0 ± 0.6 |
| LIII | 29.8 ± 2.3* | 21.5 ± 1.6 | 19.2 ± 2.3 | 29.5 ± 2.5* | 24.8 ± 2.1 | 24.8 ± 1.9 |
| LIV | 39.4 ± 2.2 | 35.1 ± 2.1 | 34.9 ± 2.2 | 35.8 ± 2.2 | 33.8 ± 1.8 | 32.0 ± 1.9 |
| LV | 40.0 ± 1.8* | 31.0 ± 2.1 | 32.0 ± 1.8 | 39.2 ± 3.1* | 33.4 ± 2.1 | 32.0 ± 2.0 |
Data show both the mean number of cells expressing UCH-L1 mRNA and the mean density of silver grains. Ipsilateral values were compared with both the contralateral side in CCI animals taken at peak behavioral change and with naive, unoperated values (
*
p < 0.05; one-way ANOVA, followed by a Dunnett's post hoc test). For all laminas analyzed, there was no significant alteration in the relative expression of UCH-L1 mRNA when comparing contralateral CCI values with naive values.