. 2002 Feb 15;22(4):1363–1372. doi: 10.1523/JNEUROSCI.22-04-01363.2002

Table 4.

Lack of effect of topical administration of the proteasome inhibitor epoxomicin on membrane translocation/activation of protein kinase C

Topical administration	Specific [³H]PDBu binding (% associated with membrane fraction)
	Naive control	CCI
	Naive control	Ipsilateral	Contralateral
Vehicle	24.0 ± 3.7	61.4 ± 6.5^4-150	46.0 ± 4.9^4-150
Epoxomicin	26.2 ± 2.9	61.0 ± 4.8^4-150	48.5 ± 6.1^4-150

After topical administration of epoxomicin or vehicle under anesthesia (as in Table 3), spinal cord segments L3–L6 were removed and hemisected before homogenization in ice-cold buffer. Samples were assayed for membrane-bound and cytosolic specific [³H]PDBu binding sites. Total recovered specific binding was in the order of 6000 dpm per sample aliquot and was similar in all conditions tested. Values are means ± SEM (n = 5).

F4-150: indicates significantly greater than naive control values (p < 0.05 by Mann–Whitney U test). PDBu, Phorbal dibutyrate.