Table 1.

Oligodeoxynucleotide probes used for in situhybridization

Target	Species	Probe position	Mismatch	Sequence of the oligonucleotide	Tm (°C)
α3	Rat	138–182	?	5′-GGACACCTCAAACTGGATGATGACTGGATGGGACACATTAGCCAC-3′	65
α4	Mouse	570–614	0	5′-GAAGTCCAGTTGGTCCACACGGCTGTGCATGCTCACCAAGTCAAT-3′	71
α5	Rat	1077–1121	5	5′-CCGAGATTTAGGTCCAgCccCacTCTCGGCTTCTTCTCTCTGAGT-3′	73
α6	Mouse	1451–1497	0	5′-GGGACAATACCAATTAAGAGTTTCCTGTGTTCCCAAGCAGTGGCTGC-3′	66
α7	Rat	1206–1250	2	5′-ATCATGTGTTGGGGAGCAGGCCAAACGgCCACAtACGACCCCAGA-3′	71
β2	Mouse	1269–1313	0	5′-TCGCATATGGTCCGCAATGAAGCGTACACCGTCCACAGCTTCCCG-3′	71
β4	Rat	1261–1305	?	5′-AGCTGACACCCTCTAATGCTTCCTGTAGATCTTCCCGGAACCTCC-3′	71
TH	Rat	1429–1468	2	5′-AGGTGTGCAGCTCATCCTGGACCCCCTCcAAGGAGCGCt-3′	79
D1	Mouse	592–638	0	5′-GCTGACGATCATAATGGCTACGGGGATGTAAAAGCTTATGAGGGAGG-3′	63
D2	Mouse	928–972	0	5′-GTTTGGCAGGACTGTCAGGGTTGCTATGTAGACCGTGGTGGGATG-3′	70
CCK	Rat	264–308	3	5′-gAAATCCATCCAGCCCATGTAGTCCCGGTCACTTATcCTgTGGCT-3′	74
PPE	Rat	386–430	3	5′-GCATCCTTCTTCATGAAagCGCCATACCTCTTGGCAAGGATCTCg-3′	67
PPT	Rat	123–167	1	5′-GGCGATTCTCTGaAGAAGATGCTCAAAGGGCTCCGGCATTGCCTC-3′	68

The melting temperature (Tm) is calculated for the most stringent wash condition (0.01 m NaCl, 0% formamide) as described byLe Novère et al. (1996). The indicated position is in reference to the A of the ATG. When the oligonucleotide was designed according to the rat cDNA sequence, and when corresponding mouse cDNA sequence was available, we indicated the number and position (lowercase characters) of the mismatches. (“?” means that the mouse cDNA sequence was not available).