Table 3.
αCtxMII inhibition of [3H]epibatidine binding on superior colliculus and striatal membranes
| Total [3H]epibatidine binding (fmol/mg protein) | αCtxMII sensitive population (fmol/mg protein) | Ki (nm) | |
|---|---|---|---|
| Wt (SC) | 173.4 ± 9.6 | 59.7 ± 3.8 | ND |
| α6−/−(SC) | 102.0 ± 6.8 | 9.7 ± 1.8 | ND |
| Wt(Str) | 57.9 ± 2.0 | 12.1 ± 1.0 | 1.4 ± 0.2 |
| α6−/−(Str) | 53.8 ± 1.7 | −1.2 ± 0.8 | NA |
Membranes were prepared from SC and striatum (Str) of Wt and α6−/− mice. αCtxMII-sensitive sites were measured using 10 μm unlabeled αCtxMII with 500 pm[3H]epibatidine. Each value represents the mean ± SEM of six separate experiments (expressed in femtomoles per milligram of protein). For Ki determination, data were fitted to a one site model (see Material and Methods). NA, Not applicable. ND, Not determined.