Table 4.

Cytisine inhibition of [³H]epibatidine binding on striatal membranes

	B1 (fmol/mg protein)	Ki1(nM)	B2 (fmol/mg protein)	Ki2 (nM)
Wt	45.9  ± 3.4	0.23  ± 0.03	12.8  ± 2.8	4.0  ± 1.3
α6−/−	56.5  ± 1.8	0.32  ± 0.03	—	—

Membranes were prepared from striatum of Wt and α6−/− mice. Membrane samples were incubated in the presence of 500 pm[³H]epibatidine with 30 pm to 3 μm unlabeled cytisine. Results were fitted to a one (α6−/−) or two (Wt) site model (see Material and Methods). Each value represents the mean ± SEM of five separate experiments.