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. 2002 Feb 15;22(4):1328–1337. doi: 10.1523/JNEUROSCI.22-04-01328.2002

Fig. 4.

Fig. 4.

Immunocytochemical labeling of synapsin, GAD65, and GABAAR, and EGFP transfection. A, Dendrites stained against synapsin (red) and GAD65 (green), with double-labeled puncta shown inyellow. B, Dendrite stained against synapsin (red) and GABAARs (green), with overlap shown inyellow. C, Apical dendritic arbor of a pyramidal neuron transfected with EGFP. D, High-magnification image of the neuron in C, showing EGFP-labeled dendrites and immunocytochemical labeling of synapsin (inred). E, Pyramidal neuron double-labeled for synapsin (red) and GABAAR (green). F, Higher-magnification view of the apical dendrite shown in E. Scale bars:A, B, D, F, 5 μm; C, 25 μm; E, 15 μm.Arrows indicate colabeling of puncta (A,B, F) or synapses made onto the EGFP-filled dendrite (C).