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. 2002 Jan 1;22(1):156–166. doi: 10.1523/JNEUROSCI.22-01-00156.2002

Fig. 5.

Fig. 5.

JNK activation is necessary for NGF-dependent apoptosis in oligodendrocytes. A, DN-JNK2 inhibits NGF-dependent activation of JNK in oligodendrocytes. Oligodendrocytes were infected with GFP control or DN-JNK2 adenovirus for 24 hr at 150 pfu/cell. Infected cells were untreated or treated with NGF for 4 hr, and the lysates were subjected to solid-phase kinase as well as IP/K assays. The presence of DN-JNK2 is shown in an HA Western blot, and the JNK protein is shown in a JNK Western blot. B, DN-JNK2 rescues oligodendrocytes from NGF-mediated apoptosis. The quantification procedure was identical to what was described in the legend of Figure 2.