Fig. 12.

The distribution of VGLUT1 and VGLUT2 in the cerebellum. A, B, Extreme density of intensely stained VGLUT1-positive puncta in the molecular layer (m), very sparse VGLUT1 puncta around somata of Purkinje cells in the Purkinje cell layer (p), and dense glomerula-like accumulation of strongly stained confluent VGLUT1 puncta in the granular layer (g) is observed. C, D, VGLUT2-ir puncta are much less dense in the molecular layer where they are arranged in a strand-like manner. VGLUT2-ir puncta forming glomerula-like structures in the glomerular layer (g) are less dense than those staining for VGLUT1. Scale bars: A,C, 500 μm; B, D, 100 μm.