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. 2002 Jan 1;22(1):142–155. doi: 10.1523/JNEUROSCI.22-01-00142.2002

Fig. 5.

Fig. 5.

The distribution of VGLUT2 and VGLUT1 in the forebrain. A, H, Punctate VGLUT2-ir is relatively abundant throughout the hypothalamus and thalamus where VGLUT1 is restricted to the hypothalamic ventral premammillary nucleus (PMV) and to parts of the thalamic nuclei including the lateral posterior thalamic nucleus (LP), dorsal lateral geniculate nucleus (DLG), and ventral posteromedial thalamic nucleus (VPM).OPT, Olivary pretectal nucleus; APTD, dorsal anterior pretectal nucleus; PrC, precommissural nucleus. VGLUT2 staining is moderate in a band of the neocortex comprising lamina IV, weak in a neocortical band comprising lamina VI, and minimal in the other neocortical layers. Intense punctate VGLUT1-ir is abundant throughout the cortex including the piriform cortex (Pir) and somewhat less strong in the neocortical band of lamina IV where moderate VGLUT2 staining accumulates. Note mutual exclusion of VGLUT1 and VGLUT2 staining in the layers of the retrosplenial granular cortex (A,H, RSG) shown at high magnification inE and L. High magnifications (G, N) from lamina IV in F andM demonstrate different densities of VGLUT1- and VGLUT2-ir puncta between immunonegative neuronal cell bodies and processes. Sparse VGLUT2-ir puncta are mostly confined to the granular layer (g) of the dentate gyrus (DG) and pyramidal layer (p) of the fields CA1, CA2, and CA3 of the hippocampus. Dense VGLUT1-ir puncta are abundant throughout the hippocampus with the exception of the granule (g) and pyramidal (p) cell layers. Areas inrectangles in A and corresponding areas on adjacent section in H are shown at high magnification in B–D andI–K, respectively. Note differential distribution and density of VGLUT1-ir and VGLUT2-ir in the oriens layer (o), pyramidal layer (p), stratum radiatum (r), and stratum lacunosum moleculare (l) of CA1 (B,I) and CA3 (C,J) and in the molecular (m), granular (g), and polymorphic (p) layer of the dentate gyrus (DG) (D, K). Note some overlap but differential density and intensity of immunostaining for VGLUT1-ir and VGLUT2-ir puncta in the posterior basomedial amygdaloid nucleus (BMP), in the lateral amygdaloid nucleus (La), and in the cortical amygdaloid nucleus (Co) as well as in the adjacent dorsal endopiriform nucleus (DEn). Note that white matter and fiber tracts are VGLUT1 and VGLUT2 negative. pc, Posterior commissure; f, fornix; fr, fasciculus retroflexus; mt, mammillothalamic tract. Scale bars:A, E, 1.5 mm;B–D, 50 μm;F–H, 50 μm; I,J, 100 μm; K, L, 50 μm; M, N, 12.5 μm.