Fig. 6.
The effect of α-SNAP on exocytosis required ATP hydrolysis and stimulation of the ATPase activity of NSF.A, Replacement of intracellular ATP with ATP-γ-S (2 mm) abolished the effect of α-SNAP (500 nm) but had no significant effect in the absence of exogenous α-SNAP.B, The rate of exocytosis was not affected by a mutant α-SNAP that does not stimulate the ATPase activity of NSF. In all experiments shown here, [Ca2+]i was buffered to ∼170 nm. For comparison, the regression line (from Fig. 2B) for control data obtained in the presence of intracellular ATP, but no exogenous α-SNAP, is also shown.