Table 1.
Compositions of pipette solutions for dialysis
| Calculated [Ca2+] | ∼0.1 nm | 50 nm | 100 nm | 170 nm | 300 nm | 500 nm | 170 nm (ATP-γ-S) |
|---|---|---|---|---|---|---|---|
| KCl | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| KAsp | 40 | 40 | 40 | 40 | 40 | 40 | 40 |
| HEPES | 40 | 40 | 40 | 40 | 40 | 40 | 40 |
| GTP | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Na2ATP | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | |
| Li4ATP-γ-S | 2.0 | ||||||
| MgCl2 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| EGTA | 20 | 20 | 20 | 20 | 20 | 20 | |
| BAPTA | 10 | ||||||
| CaCl2 | 0 | 8 | 12 | 14 | 16 | 17 | 14 |
| Indo-1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| pCa | ∼10 | 7.3 | 7.0 | 6.8 | 6.5 | 6.3 | 6.8 |
The concentration of all ingredients is listed in millimolar. All solutions were adjusted to pH 7.4 by KOH.