Fig. 1.

Decrease of dSlo channel activity upon coexpression of PKAc. dSlo currents were evoked by 300 msec depolarizing voltage steps from −80 mV in 15 mV increments, in the whole-cell voltage-clamp configuration, with 10 μmintracellular free Ca²⁺. Currents were recorded from tsA201 cells transfected with either dSlo alone (A) or dSlo and PKAc (B).C, The peak dSlo current density at +165 mV (mean ± SEM), normalized to membrane capacitance (I/Cm), is significantly lower in cells cotransfected with PKAc (p < 0.001). D, The activation time constant (τ) (mean ± SEM), from single exponential fits of dSlo currents evoked at +165 mV, is significantly increased with cotransfection of PKAc (p < 0.001).