Fig. 3.

K72E PKAc does not modulate dSlo channel activity. Whole-cell dSlo currents were elicited by 400 msec depolarizing voltage steps from −80 mV in 20 mV increments. Currents were recorded from tsA201 cells transfected with either dSlo alone (A) or dSlo and K72E PKAc (B). There is no significant difference in the peak current density (mean ± SEM) of dSlo currents recorded at +150 mV from cells transfected with dSlo alone or from cells transfected with dSlo and K72E PKAc (C) (p > 0.8). The activation time constant (mean ± SEM) of dSlo currents evoked at +150 mV is also not significantly changed with cotransfection of K72E PKAc (D) (p > 0.8).