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. 2002 May 15;22(10):3855–3863. doi: 10.1523/JNEUROSCI.22-10-03855.2002

Fig. 5.

Fig. 5.

S942 is the only target in the dSlo C-terminal domain of in vitro PKA phosphorylation.A, Schematic diagram of the dSlo channel protein shows the two regions in the dSlo C-terminal domain used to generate GST-fusion proteins. At the bottom is the amino acid sequence in the region from residues 922–956 that is critical for dSlo binding to PKAc. B, C, In vitro, PKAc phosphorylates both wild-type GST-A and GST-Cter (B,C, left lanes), but does not phosphorylate either S942A GST-A (B, right lane) or S942A GST-Cter (C, right lane). Equal amounts of wild-type and mutant GST-fusion proteins were used in these experiments.