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. 2002 May 15;22(10):3855–3863. doi: 10.1523/JNEUROSCI.22-10-03855.2002

Fig. 6.

Fig. 6.

dSlo binds to free PKAc but not to the holoenzyme. dSlo immunoprecipitates were probed on Western blots for PKAc (top panel). Cells were transfected with dSlo alone (lane 1), dSlo and wild-type (WT) PKAc, together with either control vector (lane 2), PKA regulatory subunit RIα (lane 3), or RIβ (lane 4) or dSlo and H87Q/W196R (MT) PKAc, together with RIα (lane 5), RIβ (lane 6), or control vector (lane 7). Cotransfection of RIα or RIβ abolishes the interaction between dSlo and WT PKAc but has no effect on binding of dSlo to MT PKAc (top panel). Expression of both WT and MT PKAc is similar under all transfection conditions (middle panel). Lysates were probed with anti-RI antibody for expression of RIα or RIβ (bottom panel).