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. 2002 May 15;22(10):3855–3863. doi: 10.1523/JNEUROSCI.22-10-03855.2002

Fig. 7.

Fig. 7.

Mutational analysis of the dSlo-PKAc interaction. dSlo immunoprecipitates were probed on Western blot for PKAc.A, Cells were transfected with PKAc, together with control vector (lane 1), wild-type (WT) dSlo (lane 2), dSlo deletion mutant (dSloΔ1, lane 3), S942A dSlo (lane 4), or R939A/R940A dSlo (lane 5). Deletion of 35 amino acids in dSlo abolishes its binding to PKAc (lane 3, top panel), but the dSlo mutants bind well to PKAc (lanes 4, 5, top panel). Expression of WT and mutant dSlo channels is similar under all transfection conditions (bottom panel). B, dSlo was cotransfected with wild-type (WT) PKAc (lanes 1,2), R133A PKAc (lane 3), or R133Q PKAc (lane 4). Less PKAc was detected in dSlo immunoprecipitates after incubation of cells with the membrane-permeant PKI (lane 2, top panel). Neither R133 mutation in PKAc affects its binding to dSlo (lanes 3, 4, top panel). Expression of wild-type and mutant PKAc is similar under all transfection conditions (bottom panel).