Fig. 5.
Protein nitration detected by nitrotyrosine immunostaining at P14. A, Eyes of iNOS(+/+) mice of the ischemic phase of the experiment. B, Eyes of iNOS(−/−)mice of the ischemic phase of the experiment. C, Room-air-raised iNOS(+/+) control mice. Nitrotyrosine-positive cells were found at the edge of the capillary-free, central retina of ischemic iNOS(+/+)sections in an RMG cell-like distribution in proximity to blood vessels (A). Ischemic iNOS(−/−) mice (B) and room-air-raised iNOS(+/+) mice (C) did not show any positive cells. Panel height: A–C, 240 μm.