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. 2002 May 15;22(10):4153–4162. doi: 10.1523/JNEUROSCI.22-10-04153.2002

Fig. 4.

Fig. 4.

Morphine downregulates cAMP signaling in BDNF-deficient mice. A–D, Representative traces of single-unit recordings of LC neurons in wild-type animals (A, C) and BDNF conditional mutant mice (B, D) treated with saline (A, B) or chronic morphine (C, D). The y-axis shows firing rate (spikes/10 sec); the horizontal axis represents running time (calibration: 5 min); arrows mark time period when forskolin (10 μm) was added to the perfusate. Notice decrease in firing in conditional mutant (D).Bar graph in E summarizes recording data for saline- and morphine-treated controls, and bar graphin F summarizes recording data for saline- and morphine-treated BDNF mutants. Open bars inE and F show neuronal firing rate in the absence of forskolin; black bars indicate neuronal firing rate with forskolin (Fo) added to the perfusate.G summarizes forskolin-stimulated AC enzymatic activity (picomoles per milligram of tissue per minute) in saline- and morphine-treated conditional mutants (hatched bars) and controls (diagonal line bars). Sal., Saline; Morph., morphine. Data shown as mean ± SE. *p < 0.05; **p < 0.01 (t tests).