Fig. 5.

Noradenergic neurons of BDNF-deficient mice lack dynamic regulation of TH. A, Representative Western blot showing TH immunolabeling and PKA (catalytic subunit) immunolabeling as loading control in LC of wild-type and BDNF-deficient mutant mice chronically treated with morphine (+) or sham (−) surgeries. Notice increased TH immunolabeling in morphine-treated wild type but not in morphine-treated mutant. B, Relative TH-immunoreactivity (y-axis) as determined densitometrically in mutant and wild-type mice. Bars with diagonal lines indicate drug-naïve animals (sham surgery), andhatched bars indicate morphine-treated mice. Data are expressed as mean ± SE. *p < 0.05.