Fig. 8.
Recurrent inhibitory postsynaptic currents of M-cell. A, IPSCs were monitored by whole-cell recording with a KCl-filled patch electrode (ECl, −40 mV) from an M-cell of a 4 dpf zebrafish. Each trace was averaged from seven responses. Recurrent IPSCs (asterisk) were elicited in an all-or-nothing manner associated with the preceding spike of the M-cell (arrow) evoked by brief depolarization (to +20 mV). The holding potential was −70 mV. Input resistance was 23 MΩ.B, Recurrent IPSCs (black) were completely abolished (blue) within 30 min by bathing with strychnine (1 μm)-containing solution. The currents recovered (gray) after 23 min washout.C, Confocal fluorescence image of another M-cell after injecting Lucifer yellow from the recording patch pipette. The lateral dendrite and soma of the cell were clearly observed, with the thick axon descending through the contralateral side of medulla. Scale bar, 50 μm.