Fig. 2.

Blockade of DSE by a cannabinoid antagonist, SR141716A. Averaged time courses of the changes in EPSC amplitudes induced by depolarization to 0 mV for 10 sec before (A) and after (B) treatment with 5 μm SR141716A are shown. The data shown inB were acquired at least 30 min after the bath application of the antagonist. EPSCs were evoked at 1 Hz. In each experiment, mean amplitudes of five consecutive EPSCs were calculated. Each point is the average of the mean EPSC amplitudes for the 5 sec period from five different cells. EPSC traces acquired before and after depolarization (Depo) were superimposed (right). Each trace is the average of eight consecutive EPSCs.