Average number of vesicles in the docked (active zone) pool and in the vesicle cluster, located more than one vesicle diameter from the synapse. A, Average number of vesicles in synapses from Snca+/+ andSnca−/− hippocampal neuronal cultures; n = 61 from each genotype.B, Average number of vesicles in synapses fromSnca+/+ andSnca−/− hippocampal brain samples;n = 131 from each genotype. Significance for differences in mean between Snca+/+and Snca−/−: *p= 0.026, **p = 0.00025, ***p = 0.00015, Student's two-sided t test,.