Fig. 5.

Rapsyn–GFP localization is dependent on the integrity of the microtubular network. COS-7 cells expressing rapsyn–GFP were fixed and handled for immunofluorescence microscopy using monoclonal anti-α-tubulin antibody. A, In cells starting to express rapsyn–GFP (24 hr), TGN-associated rapsyn–GFP appeared in close proximity with the MTOC (arrow).B, At later expression times (typically 2 d), rapsyn–GFP distributed as numerous organelles surrounding the MTOC and underlying the cell cortex (arrowheads).C, Confocal analysis suggested the association of rapsyn–GFP carriers with microtubules (arrows).D, Depolymerization of microtubules by nocodazole disrupted juxtanuclear and cortical distributions of rapsyn–GFP.A, B, D, DAPI staining was performed to localize the nuclei. Scale bars, 10 μm.