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Dual-label immunohistochemistry for insulin receptor-β and POMC. The top twopanelsare confocal images (5 μm optical section) of ARC neurons. Positive immunoreactivity for the insulin receptor-β is depicted ingreen (A), whereas POMC-positive immunoreactivity is depicted in red(B). C, An overlay of the above images. Greenarrows point to single-labeled neurons; yellowarrowsindicate dual-labeled neurons. Scale bar, 50 μm.
