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. 2002 Oct 15;22(20):9015–9023. doi: 10.1523/JNEUROSCI.22-20-09015.2002

Fig. 1.

Fig. 1.

Histological examination shows successful expression of transgenes in the primary visual cortex.A, The photomicrograph shows a very large number of cells expressing mCREB–GFP near the site of an injection made 4 d earlier. Scale bar, 180 μm. B, Few cells expressed mCREB–GFP ∼2 mm away from the injection. C, Darkly labeled cells stained with CREB-specific antibodies (1:1000; Upstate Biotechnology) are seen near the injection of HSV–CREB. Note the presence of electrolytic lesions (arrows) made during an electrode penetration near the injection site. D,Lightly stained cells ∼2 mm away from the injection of HSV–CREB. The immunohistochemical procedures were chosen to minimize detection of endogenous CREB and therefore underestimate the number of infected cells.