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Triple immunofluorescence for NOS-I (blue), sGCβ (green), and the presynaptic marker synaptophysin (Syn,red), in the stratum radiatum of CA1. Examples of puncta double stained for sGCβ and synaptophysin, apposed to puncta stained for NOS-I, are circled. Contrast is enhanced in the triple-labeled panel (right) to facilitate identification of multiple labeling. Scale bar, 10 μm.
