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. 2002 Oct 15;22(20):8951–8960. doi: 10.1523/JNEUROSCI.22-20-08951.2002

Fig. 5.

Fig. 5.

METH-induced neurotoxicity is mediated by DAT. Postnatally derived VMAT2 +/+ neuronal cultures were exposed to either vehicle (bar 1), 500 μm METH (bar 2), 20 μm nomifensine (bar 5), or 1 μm amfonelic acid (bar 6), or were pretreated with nomifensine or amfonelic acid 30 min before METH exposure (bars 3 and 4, respectively). After 7 d of exposure, cells were immunostained for TH and assessed as in Figure 3. Nomifensine effectively prevented METH-induced neurodegeneration (Mann–Whitney U test;p = 0.0002; U = 191;n = 18 for METH and n = 19 for METH plus nomifensine); amfonelic acid was also effective (p < 0.0001; U = 273.5;n = 18 for METH and n = 15 for METH plus amfonelic acid). *p < 0.0001 for METH; **DAT inhibitor plus METH.