Fig. 6.

Src kinase, MAP kinase, and PI3 kinase are required for L1-potentiated migration of B35 cells. Migration of B35 cells expressing no L1 or L1(+RSLE) was measured toward fibronectin for 16 hr. Cells were untreated or treated with 0.05% DMSO alone or with Src kinase inhibitor PP2 (1 μm), inactive analog PP3 (1 μm), PI3 kinase inhibitor Ly294002 (25 μm), or MEK inhibitor UO126 (10 or 20 μm). Each sample was assayed in triplicate, and experiments were repeated at least twice with similar results. *Statistically significant differences in means of treated and untreated cells using the t test (p < 0.05).