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Subcellular distribution of heterologously expressed P2X receptors in neurons. Confocal images of heterologously expressed P2X4 WT (A, B), P2X4–GFP (C), P2X2 WT (D, E), and P2X2–GFP (F) receptors in mature OB neurons.Insets,C, F, Example traces of whole-cell currents evoked by a 0.5 sec application of ATP (100 μm). Cells expressing untagged receptors were stained using anti-P2X4/Cy3 and anti-P2X2/Cy3, respectively. Scale bars: A, B, D,E, 10 μm; C, F, 5 μm.
