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P2X4 determines heteromeric P2X4/6 receptor trafficking. A, Confocal image to show the colocalization between P2X6–GFP and the ER marker calreticulin.B, Coexpression of P2X6–GFP with P2X4 WT and their colocalization with the early endosomal marker EEA-1. C, Coexpression of P2X2–GFP and P2X4 WT in HEK293 cells. Cells were stained using anti-calreticulin/Cy3 (A) or anti-P2X4/Cy5 and anti-EEA-1/Cy3 (B) and anti-P2X4/TRITC (C). Right panelsshow overlaid images (A, C). Scale bars, 10 μm.
