Fig. 1.

Candidate gene fragments identified by ddPCR. Portion of a ddPCR sequencing gel showing a ddPCR product that is expressed more highly in normal (N) cat visual cortex at 5 weeks and in dark-reared (D) cat visual cortex at 20 weeks (top arrowhead). Below it is seen as a different band that is expressed more highly in D cats at 5 weeks and N cats at 20 weeks (bottom arrowhead). In this case, both patterns of differentially expressed bands were detected by a single oligo dT-X/arbitrary primer pair. Independently isolated RNA samples from two cats at each age and rearing condition were run together (8 lanes) to help screen out false positives. The ddPCR analysis was also repeated with the same primer pair, and it confirmed reproducibility (data not shown).