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. 2002 Nov 15;22(22):9698–9707. doi: 10.1523/JNEUROSCI.22-22-09698.2002

Fig. 3.

Fig. 3.

Coronal sections showing the distribution of SK1–SK3 protein in rat brain. Low-magnification microphotographs show the overall distribution of SK1–SK3 immunoreactivity in coronal brain sections. Adjacent sections (40 μm) were stained with affinity-purified anti-SK1(12–29) (2.0 ng/μl IgG), anti-SK2(538–555) 1:6000 (crude serum), and anti-SK3(504–522) (1.3 ng/μl IgG). Nonspecific immunostaining was assessed using preimmune serum. Immune serum preadsorbed to immunogenic peptide yielded essentially identical results. 3V, Third ventricle; Amyg, amygdala, CM, centromedial thalamic nucleus;CPu, caudate putamen; Cx, neocortex;Hi, hippocampus; LD, laterodorsal thalamic nucleus; LH, lateral hypothalamic area;PV, paraventricular thalamic nucleus; Rt, reticular thalamic nucleus; VP, ventroparietal thalamic nucleus. Scale bar, 1000 μm.