Fig. 1.

Slice orientation. The diagram shows the typical orientation of the cell pairs recorded in this study. Purkinje cells for which the soma was near the surface and for which the dendrites descended into the pseudo-transverse slice were whole-cell clamped. Granule cells within the connected beam were recorded in loose cell-attached mode. The thickness of our slices (450 μm) exceeds the combined depths of the molecular and granule cell layers (220 + 180 μm; Harvey and Napper, 1988), ensuring that parallel fibers (PF) between recorded granule cells (GCs) and Purkinje cells (PC) were not cut at the bottom surface of the slice.