Fig. 2.

NN-18 and RMO-44 anti-NF-M antibodies recognize phosphorylated and nonphosphorylated NF-M, respectively, in native conformation. Mixed cell aggregates were used at 13 d of culture.A, C, Controls; B, D, dephosphorylation (Dephosphor.) by alkaline phosphatase. Cryosections were stained by immunohistochemistry against NF-M (A, B, NN-18 antibody; C, D, RMO-44 antibody). Arrow, Neuronal cell body;arrowhead, proximal axon, arising from the neuronal cell body and growing toward aggregate periphery; asterisk, peripheral zone enriched in axons. Scale bar, 100 μm.