Fig. 5.

Cholinergic axons of day 13 mixed cell aggregates are affected by NH₄Cl exposure and rescued by Cr cotreatment. Aggregates were treated from days 5 to 13 with or without (in mm): 5 NH₄Cl, 1 Cr, or both. Immunohistochemistry against ChAT (A–D) and GAD 65/67 kDa (E, F) was performed. A, E, Controls; B, F, NH₄Cl;C, Cr; D, NH₄Cl + Cr. The dense peripheral axonal zone is indicated by anasterisk. Scale bar, 100 μm.