Fig. 5.
Effects of Bim suppression on Smac release and CEC death. CECs were cultured in the presence or absence of bimsense or antisense oligonucleotide with or without Aβ25−35 treatment for 24 hr.A, A representative immunoblot demonstrates thatbim antisense oligonucleotide reduced bimexpression in CECs after Aβ25−35exposure. Sense oligonucleotide treatment had no effect.B, bim antisense oligonucleotide reduced mitochondrial Smac release in CECs after Aβ25–35treatment, as demonstrated in this representative Western blot, and increased CEC survival, assessed by MTT assay (C) and LDH release (D). Data are expressed as mean ± SD from three separate experiments in quadruplicate.Control, Control without oligonucleotide treatment;AS, antisense oligonucleotide; S, sense oligonucleotide. **p < 0.05 versus control group.