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. 2019 Sep 17;10:1124. doi: 10.3389/fpls.2019.01124

Figure 2.

Figure 2

Knockout of NRAMP6 impaired lateral root growth under Fe-deficient conditions. (A) The T-DNA was inserted in the 9th intron of the NRAMP6 gene. (B) Relative NRAMP6 transcript levels in wild-type, nramp6-1, and three complemented lines OxNRAMP6 #1, OxNRAMP6 #2, and OxNRAMP6 #3, assessed by quantitative RT-PCR. Actin2 was used as an internal control. Data represent the mean ± SD (n = 3). (C) Phenotypic analysis of the indicated plants in response to Fe-deficient conditions. WT, nramp6-1, and complemented lines were sown on a one-half-strength Hoagland agar medium either in Fe-replete conditions (20µM Fe[III]-EDTA) or in Fe-free (no added Fe) conditions as indicated. Plants were grown vertically for 14 days. (D-H) Shoot fresh weight (D), root fresh weight (E), primary root length (F), average lateral root length (G), lateral root density (H). Data are means ± SD. Means with different letters are significantly different (P < 0.05, Tukey’s test).