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. 2019 Sep 17;10:1124. doi: 10.3389/fpls.2019.01124

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Copyright © 2019 Li, Wang, Zheng, Li, Zhou, Li, Gu, Xu, Lu, Chen and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the NRAMP6 mutation on Fe accumulation and chlorophyll content in Arabidopsis thaliana. (A) Phenotypes of plants grown in hydroponic conditions. WT, nramp6-1, and complemented lines were sown on agar plates for 2 weeks and then transferred to hydroponic cultivation for 1 week in Fe-replete conditions followed by 7 d in Fe-replete or Fe-deficient conditions. (B-E) Dry weight of roots (B) and shoots (C). Fe contents in roots and young leaves (three to four top leaves), and old leaves (the rest of leaves) (D). Chlorophyll levels of WT, nramp6-1, and three overexpression lines (E). Data represent the mean ± SD of three biological replicates. Means with different letters indicate a significant difference at P < 0.05 using Tukey’s test.