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Expression of AtNRAMP6 increased the sensitivity of Δccc1. Wild-type and mutant yeast strains containing the empty vector or AtNRAMP6 were spotted onto synthetic dropout (SD)-Ura plates with BPDS or metal supplementation as indicated. (A) AtNRAMP6 fails to complement the phenotype of the yeast mutant Δfet3fet4 without supplemented with Fe. (B) The expression of AtNRAMP6 exacerbated the sensitivity of the yeast mutant Δccc1 to excessive Fe.
