Fig. 5.

Chronic rotenone treatment sensitized cells to H₂O₂-induced death and oxidative protein damage. Cells were grown in medium supplemented with 5 nmrotenone for 1–4 weeks before exposure to 300 μmH₂O₂. Cell death was then monitored over 24 hr. Cells treated with rotenone for 1 week (A) showed responses to H₂O₂ similar to those of control cells. However, after exposure to rotenone for 4 weeks (B), cells were markedly sensitized to H₂O₂. Results show mean ± SEM of three independent experiments at each time point. *p < 0.05. A.U., Arbitrary units.