Fig. 3.

Vimentin-dependent Hm3-GFAP filament formation in the mouse brain. Sections of the hippocampus and cerebellum from adult animal brains of indicated genotypes were stained with anti-GFAP, anti-vimentin, or anti-S100B antibody.B–D, F–H, J–L, andN–P were counterstained with diluted hematoxylin. Note that Hm3-GFAP does not form filaments in the cerebellar cortex in the absence of vimentin. py, Pyramidal cell layer;gr, granule cell layer; ml, molecular layer. Scale bar: A, B, E,F, I, J, M,N, 200 μm; C, D,G, H, K, L,O, P, 100 μm.