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Abnormal aggregate formation of Hm3-GFAP in vimentin-deficient astrocytes. Primary cultured astrocytes were prepared from wild-type (A),Gfaphm3/hm3 (B),Vim−/− (C), andGfaphm3/hm3:Vim−/−(D) embryos. Glial filaments were immunostained with anti-GFAP antibody. The filamentous structures were sparse and irregularly arrayed in the absence of vimentin (asterisks in C and D). Dot-like structures were observed inGfaphm3/hm3:Vim−/−astrocytes (arrowheads in D). Scale bar, 50 μm.
